4-Amino-5H-pyrazolo[3,4-d]pyrimidin-6(7H)-one CAS 5472-41-3

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 4-Amino-5H-pyrazolo[3,4-d]pyrimidin-6(7H)-one.

4-Amino-5H-pyrazolo[3,4-d]pyrimidin-6(7H)-one (CAS 5472-41-3), molecular formula C5H5N5O and molecular weight 151.13 g/mol, is a CATO reference standard with documented purity and traceability.

4-Amino-5H-pyrazolo[3,4-d]pyrimidin-6(7H)-one (C5H5N5O) contains an amide group, a hydroxyl group, an ether linkage. Its physicochemical properties are representative of its class; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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