Fluorescein Diacetate CAS 596-09-8
| CAS No. | 596-09-8 |
| Molecular Formula | C24H16O7 |
| Molecular Weight | 416.38 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Compared with other compounds in its class, the molecular weight of 416.38 g/mol gives Fluorescein Diacetate distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
CATO supplies Fluorescein Diacetate (CAS 596-09-8), a certified reference material (molecular formula C24H16O7, MW 416.38 g/mol) for analytical method development and quality control.
The molecular framework of Fluorescein Diacetate (C24H16O7) is characterized by a flavonoid core, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the polarity and ionization state of its functional groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C24H16O7 |
| Molecular Weight | 416.38 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >95% |
| Storage | Store at room temperature, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, acetonitrile, DMSO, water (slightly soluble) |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 17.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |
Related Fluorescein Standards
- Fluorescein Impurity 10 (CAS CATO-C4X-290010)
- Fluorescein Impurity 8 (CAS CATO-C4X-29008)
- Fluorescein Impurity 9 (CAS CATO-C4X-29009)
- Fluorescein 6-Maleimide (CAS 161598-40-9)
- Fluorescein<I>o</I>-acrylate (CAS 193419-86-2)
- 76863-28-0 Fluorescein-5-thiosemicarbazide MFCD04221206 N-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-hydrazinecarbothioamide (CAS 76863-28-0)
- Fluorescein Impurity 12 (CAS CATO-C4X-290012)
- Fluorescein-O'-acetic Acid (CAS 233759-98-3)
Recommended Standards
- Tegoprazan impurity 49 (CAS CATO-C4X-229749)
- Daidzin (CAS 552-66-9)
- Tegoprazan impurity 20 (CAS CATO-C4X-229720)
- Puerarin (CAS 3681-99-0)
- Diethyl 2-aminomaleate (CAS 86319-87-1)
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- N-[(2-Chloro-4-fluorophenyl)methyl]guanidine (CAS 1246608-84-3)
- 3-[(Pyridin-2-yl)methyl]-3,4-dihydro-1,2,3-benzotriazin-4-one (CAS 626224-23-5)
- 2-Mercapto-7-methyl-3-phenyl-5,6,7,8-tetrahydrobenzo-[4,5]thieno[2,3-d]pyrimidin-4(3H)-one (CAS 299919-85-0)
- Quinolone analog 11 (CAS 950726-39-3)
- 3-Amino-5-(methylthio)-N-(p-tolyl)-4H-1,2,4-triazole-4-carboxamide (CAS 338391-91-6)
- Ethyl 2-(1-acetylpiperidin-4-yl)acetate (CAS 78056-59-4)
- 4-(Furan-2-yl)pyrrolidin-2-one (CAS 88221-11-8)
- D-Ser32-Tirzepatide (CAS CATO-CPTD788677)
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