5α-CHOLESTAN-2β-OL CAS 604-17-1

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 5α-CHOLESTAN-2β-OL.

5α-CHOLESTAN-2β-OL (CAS 604-17-1) is a high-purity analytical reference standard with the molecular formula C27H48O and a molecular weight of 388.67 g/mol.

The molecular framework of 5α-CHOLESTAN-2β-OL (C27H48O) is characterized by a steroid core, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 5α-CHOLESTAN-2β-OL meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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