4-Chloro-3-methoxy-7-nitro-1H-isochromen-1-one CAS 62252-25-9

Description

The molecular framework of 4-Chloro-3-methoxy-7-nitro-1H-isochromen-1-one (C10H6ClNO5) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

CATO supplies 4-Chloro-3-methoxy-7-nitro-1H-isochromen-1-one (CAS 62252-25-9), a certified reference material (molecular formula C10H6ClNO5, MW 255.61 g/mol) for analytical method development and quality control.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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