3-(4-Chlorophenyl)-5-(4-nitrophenyl)-4-phenyl-4H-1,2,4-triazole CAS 62507-59-9

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 3-(4-Chlorophenyl)-5-(4-nitrophenyl)-4-phenyl-4H-1,2,4-triazole.

3-(4-Chlorophenyl)-5-(4-nitrophenyl)-4-phenyl-4H-1,2,4-triazole (CAS 62507-59-9), molecular formula C20H13ClN4O2 and molecular weight 376.80 g/mol, is a CATO reference standard with documented purity and traceability.

Based on its molecular formula C20H13ClN4O2, 3-(4-Chlorophenyl)-5-(4-nitrophenyl)-4-phenyl-4H-1,2,4-triazole contains a steroid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 376.80 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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