(3β)-3,25-Dihydroxycholest-5-en-7-one CAS 64907-23-9
| CAS No. | 64907-23-9 |
| Molecular Formula | C27H44O3 |
| Molecular Weight | 416.64 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
(3β)-3,25-Dihydroxycholest-5-en-7-one (CAS 64907-23-9), molecular formula C27H44O3 and molecular weight 416.64 g/mol, is a CATO reference standard with documented purity and traceability.
Structurally, (3β)-3,25-Dihydroxycholest-5-en-7-one (C27H44O3) contains a steroid core, a hydroxyl group, an ether linkage. The molecule exhibits a degree of unsaturation (DBE) of 6.0, corresponding to approximately 6 ring(s) or double-bond equivalent(s). With a molecular weight of 416.64 g/mol, it falls into the moderately-high-molecular-weight range. The high carbon-to-hydrogen ratio imparts good lipophilicity, allowing liquid-liquid extraction with non-polar solvents such as n-hexane or ethyl acetate.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C27H44O3 |
| Molecular Weight | 416.64 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at room temperature, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in chloroform, dichloromethane, ethyl acetate, DMSO |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1975 |
Recommended Standards
- (2E,4E,6E,8E,10E,12E,14E,16E,18E)-2,6,10,15,19,23-Hexamethyltetracosa-2,4,6,8,10,12,14,16,18,22-decaenal (CAS 59057-70-4)
- 19-Carboxy Cholesterol (CAS 26319-96-0)
- (24S)-Secalciferol (CAS 55700-58-8)
- 1α,25-Dihydroxy-7-dehydrocholesterol (CAS 61954-91-4)
- (4-(Difluoromethoxy)-3-fluoropyridin-2-yl)methanamine hydrochloride (CAS 2231673-38-2)
- 4,4-Bis(fluoromethyl)cyclohexan-1-one (CAS 1257050-71-7)
- (3S)-3-amino-3-(4-tert-butylphenyl)propanoic acid hydrochloride (CAS 1354970-50-5)
- 938458-94-7 (Piperidin-4-yl)(thiophen-2-yl)methanol DTXSID80640544 (CAS 938458-94-7)
- 5-(1-Imino-3-phenylallyl)-4,7-dimethoxybenzofuran-6-ol (CAS 61340-68-9)
- Others Impurity 85 (CAS 731016-26-5)
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- 6-Amino-4-chloronicotinaldehyde (CAS 1260664-16-1)
- 3,6-Dichloro-2,4-dimethylpyridine (CAS 1639373-35-5)
- Liraglutide Impurity 19 TFA Salt (CAS CATO-C4X-199219)
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