4-Chloro-3-(4-(4-(dimethylamino)benzylidene)-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid CAS 65036-66-0
| CAS No. | 65036-66-0 |
| Molecular Formula | C19H18ClN3O4S |
| Molecular Weight | 419.88 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
4-Chloro-3-(4-(4-(dimethylamino)benzylidene)-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid (CAS 65036-66-0), molecular formula C19H18ClN3O4S and molecular weight 419.88 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of 4-Chloro-3-(4-(4-(dimethylamino)benzylidene)-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid (C19H18ClN3O4S) is characterized by a flavonoid core, an amide group, a sulfone group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
As a certified reference material (CRM), 4-Chloro-3-(4-(4-(dimethylamino)benzylidene)-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C19H18ClN3O4S |
| Molecular Weight | 419.88 g/mol |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 12.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1975 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 3 |
| Sulfur Atoms | 1 |
Related Chloro Standards
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Recommended Standards
- Ethyl 3-(((((4-chlorobenzyl)oxy)amino)methylene)amino)-4-methoxythieno[2,3-b]pyridine-2-carboxylate (CAS 341966-57-2)
- 125305-20-6 2-(2,2-Dimethyl-1-oxopropoxy)benzoic Acid Ethyl Ester 2-O-tert-Butoxycarbonyl-benzoic Acid Ethyl Ester (CAS 125305-20-6)
- 2-((4-Amino-5-(4-chlorophenyl)-4H-1,2,4-triazol-3-yl)thio)-N-(3,5-dimethoxyphenyl)acetamide (CAS 568548-60-7)
- PF-03882845 (CAS 1023650-66-9)
- N-methoxy-N,4-dimethylthiazole-5-carboxamide (CAS 1343082-83-6)
- Ruthenium, [[1,1'-binaphthalene]-2,2'-diylbis[diphenylphosphine-κP]]dichloro(1,2-ethanediamine-κN,κN')- (CAS 899821-59-1)
- 1-((5-Bromothiophen-2-yl)sulfonyl)-1,4-diazepane hydrochloride (CAS 1365836-42-5)
- 2-(4-(Benzyl(ethyl)amino)piperidin-1-yl)acetic acid (CAS 1353951-40-2)
- 3-Allyl-2-((2-chloro-6-fluorobenzyl)thio)-6-(trifluoromethyl)pyrimidin-4(3H)-one (CAS 866143-19-3)
- L-Arginine--3-methyl-2-oxopentanoic acid (CAS 72090-04-1)
- Cyclohexyl(3,5-difluorophenyl)methanone (CAS 898769-58-9)
- S-Methyl-S-2-naphthalenylsulfoximine (CAS 952022-22-9)
- 3-Chloro-2-fluoro-6-nitrophenol (CAS 82419-40-7)
- D-[Phe6]-Semaglutide (CAS CATO-C4X-2226213)
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