1-(4-Nitrophenyl)-1h,4h,5h-pyrazolo[3,4-d]pyrimidin-4-one CAS 65973-99-1

Purity 97%+In StockPharmaceutical Standards
CAS No.65973-99-1
Molecular FormulaC11H7N5O3
Molecular Weight257.20 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

1-(4-Nitrophenyl)-1h,4h,5h-pyrazolo[3,4-d]pyrimidin-4-one CAS 65973-99-1 chemical structure

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

CATO supplies 1-(4-Nitrophenyl)-1h,4h,5h-pyrazolo[3,4-d]pyrimidin-4-one (CAS 65973-99-1), a certified reference material (molecular formula C11H7N5O3, MW 257.20 g/mol) for analytical method development and quality control.

Owing to an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage, 1-(4-Nitrophenyl)-1h,4h,5h-pyrazolo[3,4-d]pyrimidin-4-one is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 257.20 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

As a certified reference material (CRM), 1-(4-Nitrophenyl)-1h,4h,5h-pyrazolo[3,4-d]pyrimidin-4-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC11H7N5O3
Molecular Weight257.20 g/mol
AppearancePale yellow to yellow crystalline powder
Purity97%+
StorageStore at 2-8°C, protect from light, keep sealed and dry
Solubility (reference)Soluble in methanol, ethanol, DMSO
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)11.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1975
Nitrogen Atoms5

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