5-Oxo-2,5-dihydro-1H-pyrazole-3-carbohydrazide CAS 66483-27-0
| CAS No. | 66483-27-0 |
| Molecular Formula | C4H6N4O2 |
| Molecular Weight | 142.12 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
As a certified reference material (CRM), 5-Oxo-2,5-dihydro-1H-pyrazole-3-carbohydrazide meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
5-Oxo-2,5-dihydro-1H-pyrazole-3-carbohydrazide (CAS 66483-27-0), molecular formula C4H6N4O2 and molecular weight 142.12 g/mol, is a CATO reference standard with documented purity and traceability.
5-Oxo-2,5-dihydro-1H-pyrazole-3-carbohydrazide (C4H6N4O2) contains an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
Physical & Chemical Properties
| Molecular Formula | C4H6N4O2 |
| Molecular Weight | 142.12 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 4.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1975 |
| Nitrogen Atoms | 4 |
Related Oxo Standards
- 5-Oxo-4,5-dihydro-1H-pyrazole-4-carbohydrazide (CAS 395662-74-5)
- 2-(5-Oxo-4,5-dihydro-1h-pyrazol-3-yl)acetic acid (CAS 860409-48-9)
- methyl 3,6-dihydro-2H-pyran-4-carboxylate (CAS 105772-14-3)
- Methyl 3,4-Dihydro-2H-pyran-6-carboxylate (CAS 129201-92-9)
- 2-(Dihydro-2H-pyran-4(3H)-ylidene)acetic Acid (CAS 130312-01-5)
- 2-(3,6-Dihydro-2H-pyran-4-yl)acetic acid (CAS 632328-42-8)
- 2-Methyl-4,5-dihydro-furan-3-carboxylicacidmethylester (CAS 32595-98-5)
- 6-Methyl-3,4-dihydro-2H-pyran-5-carboxylic acid (CAS 5399-21-3)
Recommended Standards
- 5-Nitro-1,4-dihydropyrimidin-4-amine (CAS 84928-80-3)
- 5-Nitro-1,2-dihydropyrimidin-2-amine (CAS 84928-78-9)
- 6-(Methylamino)-2,3,4,5-tetrahydro-1,2,4-triazine-3,5-dione (CAS 64842-42-8)
- 5-Methyl-4-nitro-1h-pyrazol-3-amine (CAS 76143-53-8)
- (2E)-3-(4-{[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy}phenyl)-1-phenylprop-2-en-1-one (CAS 1025208-97-2)
- Gemfibrozil Isobutyl Ester (CAS 149105-26-0)
- 3-Amino-5-bromo-N-ethylbenzamide (CAS 1373233-48-7)
- 3’-Bromo-5’-chloro-2’-hydroxyacetophenone (CAS 59443-15-1)
- 5-Hydroxy-7-methylindoline-2,3-dione (CAS 1041857-41-3)
- n-(2-(Dimethylamino)-2-(thiophen-2-yl)ethyl)isobutyramide (CAS 1215717-31-9)
- 2-(5-Chloro-2-methyl-3-nitrophenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS 2377607-14-0)
- Ozanimod Impurity 28 (CAS CATO-C4X-290228)
- 1-Azaspiro[4.6]undecane hydrochloride (CAS 1432678-60-8)
- Polyphemusin II-Derived Peptide (CAS 229030-20-0)
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