4-((4-Methoxy-N-(4-methylbenzyl)benzamido)methyl)-3-nitrobenzoic acid CAS 672310-25-7

Description

Compared with other compounds in its class, the molecular weight of 434.44 g/mol gives 4-((4-Methoxy-N-(4-methylbenzyl)benzamido)methyl)-3-nitrobenzoic acid distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

4-((4-Methoxy-N-(4-methylbenzyl)benzamido)methyl)-3-nitrobenzoic acid (CAS 672310-25-7), molecular formula C24H22N2O6 and molecular weight 434.44 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 4-((4-Methoxy-N-(4-methylbenzyl)benzamido)methyl)-3-nitrobenzoic acid (C24H22N2O6) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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