(3β,22E)-3,25-Dihydroxy-cholesta-5,22-dien-24-one 3-Acetate CAS 69425-74-7
| CAS No. | 69425-74-7 |
| Molecular Formula | C29H44O4 |
| Molecular Weight | 456.66 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
(3β,22E)-3,25-Dihydroxy-cholesta-5,22-dien-24-one 3-Acetate (CAS 69425-74-7), molecular formula C29H44O4 and molecular weight 456.66 g/mol, is a CATO reference standard with documented purity and traceability.
Based on its molecular formula C29H44O4, (3β,22E)-3,25-Dihydroxy-cholesta-5,22-dien-24-one 3-Acetate contains a steroid core, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 456.66 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The high carbon-to-hydrogen ratio imparts good lipophilicity, allowing liquid-liquid extraction with non-polar solvents such as n-hexane or ethyl acetate.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), (3β,22E)-3,25-Dihydroxy-cholesta-5,22-dien-24-one 3-Acetate meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C29H44O4 |
| Molecular Weight | 456.66 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at room temperature, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in chloroform, dichloromethane, ethyl acetate, DMSO |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 8.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1976 |
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