| CAS No. | 70815-12-2 |
| Molecular Formula | C32H28N2O5 |
| Molecular Weight | 520.58 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 6'-(Diethylamino)-3'-methyl-3-oxo-2'-(phenylamino)-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid.
CATO supplies 6'-(Diethylamino)-3'-methyl-3-oxo-2'-(phenylamino)-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid (CAS 70815-12-2), a certified reference material (molecular formula C32H28N2O5, MW 520.58 g/mol) for analytical method development and quality control.
6'-(Diethylamino)-3'-methyl-3-oxo-2'-(phenylamino)-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid (C32H28N2O5) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
| Molecular Formula | C32H28N2O5 |
| Molecular Weight | 520.58 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 20.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1977 |
| Nitrogen Atoms | 2 |