| CAS No. | 727712-80-3 |
| Molecular Formula | C16H25N3O3 |
| Molecular Weight | 307.39 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
As a certified reference material (CRM), 3-(2-(Azepan-1-yl)-2-oxoethyl)-1,3-diazaspiro[4.5]decane-2,4-dione meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
3-(2-(Azepan-1-yl)-2-oxoethyl)-1,3-diazaspiro[4.5]decane-2,4-dione (CAS 727712-80-3), molecular formula C16H25N3O3 and molecular weight 307.39 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of 3-(2-(Azepan-1-yl)-2-oxoethyl)-1,3-diazaspiro[4.5]decane-2,4-dione (C16H25N3O3) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
| Molecular Formula | C16H25N3O3 |
| Molecular Weight | 307.39 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1986 |
| Nitrogen Atoms | 3 |