2',7'-Dichlorofluorescein CAS 76-54-0
| CAS No. | 76-54-0 |
| Molecular Formula | C20H10Cl2O5 |
| Molecular Weight | 401.20 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The occurrence of 2',7'-Dichlorofluorescein is closely related to a flavonoid core, a hydroxyl group, an ether linkage, chlorine substitution in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.
2',7'-Dichlorofluorescein (CAS 76-54-0), molecular formula C20H10Cl2O5 and molecular weight 401.20 g/mol, is a CATO reference standard with documented purity and traceability.
2',7'-Dichlorofluorescein (C20H10Cl2O5) contains a flavonoid core, a hydroxyl group, an ether linkage, chlorine substitution. Halogen substitution raises its density and boiling point, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
CATO manufactures 2',7'-Dichlorofluorescein under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C20H10Cl2O5 |
| Molecular Weight | 401.20 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor, low volatility) |
| Purity | >95% |
| Storage | Store at room temperature, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, acetonitrile, DMSO, water (slightly soluble) |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 15.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |
| Halogen Content | Cl-containing |
Related Dichlorofluorescein Standards
Recommended Standards
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- 1-(5-Methylpyridin-2-yl)piperidin-3-amine hydrochloride (CAS 1185312-35-9)
- N'-[(1E)-(2-Chloro-6-fluorophenyl)methylidene]-2-(1H-pyrrol-1-yl)benzohydrazide (CAS 347314-45-8)
- 1-(3-Chloro-5-(chloromethyl)phenyl)ethan-1-one (CAS 1393546-75-2)
- Liraglutide Impurity 14 TFA Salt (CAS CATO-C4X-199214)
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