| CAS No. | 76-59-5 |
| Molecular Formula | C27H28Br2O5S |
| Molecular Weight | 624.38 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
CATO manufactures Bromothymol Blue under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Bromothymol Blue (CAS 76-59-5) is a high-purity analytical reference standard with the molecular formula C27H28Br2O5S and a molecular weight of 624.38 g/mol.
The molecular framework of Bromothymol Blue (C27H28Br2O5S) is characterized by a steroid core, a sulfone group, a hydroxyl group, an ether linkage, bromine substitution. In reversed-phase chromatography, its retention is governed primarily by the polarity and ionization state of its functional groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.
Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.
| Molecular Formula | C27H28Br2O5S |
| Molecular Weight | 624.38 g/mol |
| Purity | >85% |
| Storage | Store at room temperature, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, acetonitrile, DMSO, water (slightly soluble) |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 13.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |
| Halogen Content | Br-containing |
| Sulfur Atoms | 1 |