| CAS No. | 81256-82-8 |
| Molecular Formula | C24H21ClFN3O7 |
| Molecular Weight | 517.89 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Compared with other compounds in its class, the molecular weight of 517.89 g/mol gives (4-Hydroxymidazolam)-β-D-glucuronide distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
(4-Hydroxymidazolam)-β-D-glucuronide (CAS 81256-82-8), molecular formula C24H21ClFN3O7 and molecular weight 517.89 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of (4-Hydroxymidazolam)-β-D-glucuronide (C24H21ClFN3O7) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage, fluorine/chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.
As a certified reference material (CRM), (4-Hydroxymidazolam)-β-D-glucuronide meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C24H21ClFN3O7 |
| Molecular Weight | 517.89 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor, low volatility) |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 15.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1980 |
| Halogen Content | F/Cl-containing |
| Nitrogen Atoms | 3 |