(2E)-1-(2-Methoxyphenyl)-3-(3-nitrophenyl)prop-2-en-1-one CAS 858644-41-4

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

(2E)-1-(2-Methoxyphenyl)-3-(3-nitrophenyl)prop-2-en-1-one (CAS 858644-41-4), molecular formula C16H13NO4 and molecular weight 283.28 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of (2E)-1-(2-Methoxyphenyl)-3-(3-nitrophenyl)prop-2-en-1-one (C16H13NO4) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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