| CAS No. | 866009-71-4 |
| Molecular Formula | C21H21N3O3S2 |
| Molecular Weight | 427.54 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
N-{2-[N-tert-butyl-N'-(thiophene-2-carbonyl)hydrazinecarbonyl]phenyl}thiophene-2-carboxamide (CAS 866009-71-4), molecular formula C21H21N3O3S2 and molecular weight 427.54 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of N-{2-[N-tert-butyl-N'-(thiophene-2-carbonyl)hydrazinecarbonyl]phenyl}thiophene-2-carboxamide (C21H21N3O3S2) is characterized by a steroid core, an amide group, a sulfone group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
CATO manufactures N-{2-[N-tert-butyl-N'-(thiophene-2-carbonyl)hydrazinecarbonyl]phenyl}thiophene-2-carboxamide under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
| Molecular Formula | C21H21N3O3S2 |
| Molecular Weight | 427.54 g/mol |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 13.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1987 |
| Nitrogen Atoms | 3 |
| Sulfur Atoms | 2 |