| CAS No. | 866049-41-4 |
| Molecular Formula | C20H17N3O4 |
| Molecular Weight | 363.37 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Compared with other compounds in its class, the molecular weight of 363.37 g/mol gives Methyl 4-(4-oxo-3-(o-tolylcarbamoyl)pyridazin-1(4H)-yl)benzoate distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
Methyl 4-(4-oxo-3-(o-tolylcarbamoyl)pyridazin-1(4H)-yl)benzoate (CAS 866049-41-4) is a high-purity analytical reference standard with the molecular formula C20H17N3O4 and a molecular weight of 363.37 g/mol.
The molecular framework of Methyl 4-(4-oxo-3-(o-tolylcarbamoyl)pyridazin-1(4H)-yl)benzoate (C20H17N3O4) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
| Molecular Formula | C20H17N3O4 |
| Molecular Weight | 363.37 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1987 |
| Nitrogen Atoms | 3 |