| CAS No. | 868272-85-9 |
| Molecular Formula | C12H14N4 |
| Molecular Weight | N/A |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Compared with other compounds in its class, the molecular weight of 268.75 g/mol gives 3,3'-Diaminobenzidine (tetrahydrochloride hydrate) distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
3,3'-Diaminobenzidine (tetrahydrochloride hydrate) (CAS 868272-85-9), molecular formula C12H14N4HClH2O and molecular weight 268.75 g/mol, is a CATO reference standard with documented purity and traceability.
Based on its molecular formula C12H14N4HClH2O, 3,3'-Diaminobenzidine (tetrahydrochloride hydrate) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 268.75 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 3,3'-Diaminobenzidine (tetrahydrochloride hydrate) meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C12H14N4 4*HCl H2O |
| Molecular Weight | 268.75 g/mol |
| Purity | >95% |
| Storage | Store at 2-8°C, protect from light, keep tightly closed to prevent evaporation |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1987 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 4 |