3,3'-Diaminobenzidine (tetrahydrochloride hydrate) CAS 868272-85-9

Purity >95%In StockPharmaceutical Standards
CAS No.868272-85-9
Molecular FormulaC12H14N4
Molecular WeightN/A
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

3,3'-Diaminobenzidine (tetrahydrochloride hydrate) CAS 868272-85-9 chemical structure

Description

Compared with other compounds in its class, the molecular weight of 268.75 g/mol gives 3,3'-Diaminobenzidine (tetrahydrochloride hydrate) distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

3,3'-Diaminobenzidine (tetrahydrochloride hydrate) (CAS 868272-85-9), molecular formula C12H14N4HClH2O and molecular weight 268.75 g/mol, is a CATO reference standard with documented purity and traceability.

Based on its molecular formula C12H14N4HClH2O, 3,3'-Diaminobenzidine (tetrahydrochloride hydrate) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 268.75 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 3,3'-Diaminobenzidine (tetrahydrochloride hydrate) meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC12H14N4 4*HCl H2O
Molecular Weight268.75 g/mol
Purity>95%
StorageStore at 2-8°C, protect from light, keep tightly closed to prevent evaporation
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light
Degree of Unsaturation (DBE)6.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1987
Halogen ContentCl-containing
Nitrogen Atoms4

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