Cholecalciferol Impurity 32 CAS 89231-92-5
| CAS No. | 89231-92-5 |
| Molecular Formula | C27H44O2 |
| Molecular Weight | 400.64 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Cholecalciferol Impurity 32 (CAS 89231-92-5) is a certified pharmaceutical impurity reference standard with the molecular formula C27H44O2 and a molecular weight of 400.64 g/mol.
Cholecalciferol Impurity 32 (C27H44O2) contains a steroid core, a hydroxyl group, an ether linkage. Its high carbon-to-hydrogen ratio confers good lipid solubility, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The high carbon-to-hydrogen ratio imparts good lipophilicity, allowing liquid-liquid extraction with non-polar solvents such as n-hexane or ethyl acetate.
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
Physical & Chemical Properties
| Molecular Formula | C27H44O2 |
| Molecular Weight | 400.64 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at room temperature, protect from light |
| Solubility (reference) | Soluble in chloroform, dichloromethane, ethyl acetate, DMSO |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1982 |
Related Cholecalciferol Standards
- Cholecalciferol impurity 16 (CAS 86307-40-6)
- Cholecalciferol Impurity 28 (CAS CATO-C4X-158628)
- Cholecalciferol Impurity 26 (CAS CATO-C4X-158626)
- Cholecalciferol-d6 (Vitamin D3-d6) (CAS 118584-54-6)
- Cholecalciferol impurity 18 (CAS 2227309-94-4)
- trans-Cholecalciferol (CAS 1260149-80-1)
- Cholecalciferol EP Impurity A (CAS 22350-41-0)
- Cholecalciferol Impurity 15 (CAS 42607-12-5)
Recommended Standards
- Calcitriol Impurity 61 (CAS CATO-C4X-151061)
- 7,8-Epoxy-vitamin D3 (CAS 89231-90-3)
- Calcitriol Impurity 33 (CAS 104651-70-9)
- Calcifediol Impurity 1 (CAS 36149-00-5)
- Fluconazole Impurity 33 (CAS CATO-C4X-101933)
- Bifendate impurity 10 (CAS CATO-C4X-255510)
- Nintedanib Impurity 94 (CAS CATO-C4X-181494)
- (R)-5-(Hydroxymethyl)oxazolidin-2-one (CAS 97859-49-9)
- 1-(2,4-Difluorophenyl)-5-methyl-1h-pyrazole-3-carboxylic acid (CAS 1274470-76-6)
- 2-(Naphthalen-1-yloxy)propanehydrazide (CAS 330177-33-8)
- methyl azepane-2-carboxylate (CAS 5228-33-1)
- N-Ethyl-N-phenylbenzamide (CAS 16466-44-7)
- 4-Fluoro-3'-(trifluoromethoxy)biphenyl-3-methanol (CAS 1261525-52-3)
- Semaglutide impurity 214 (CAS CATO-C4X-2226214)
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