(2′R,3S,4′S,5′R)-6-Chloro-4′-(3-chloro-2-fluorophenyl)-N-[(3S)-3,4-dihydroxybutyl]-2′-(2,2-dimethylpropyl)-1,2-dihydro-2-oxospiro[3H-indole-3,3′-pyrrolidine]-5′-carboxamide CAS 908027-40-7

Description

Compared with other compounds in its class, the molecular weight of 552.47 g/mol gives (2′R,3S,4′S,5′R)-6-Chloro-4′-(3-chloro-2-fluorophenyl)-N-[(3S)-3,4-dihydroxybutyl]-2′-(2,2-dimethylpropyl)-1,2-dihydro-2-oxospiro[3H-indole-3,3′-pyrrolidine]-5′-carboxamide distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

(2′R,3S,4′S,5′R)-6-Chloro-4′-(3-chloro-2-fluorophenyl)-N-[(3S)-3,4-dihydroxybutyl]-2′-(2,2-dimethylpropyl)-1,2-dihydro-2-oxospiro[3H-indole-3,3′-pyrrolidine]-5′-carboxamide (CAS 908027-40-7) is a high-purity analytical reference standard with the molecular formula C27H32Cl2FN3O4 and a molecular weight of 552.47 g/mol.

(2′R,3S,4′S,5′R)-6-Chloro-4′-(3-chloro-2-fluorophenyl)-N-[(3S)-3,4-dihydroxybutyl]-2′-(2,2-dimethylpropyl)-1,2-dihydro-2-oxospiro[3H-indole-3,3′-pyrrolidine]-5′-carboxamide (C27H32Cl2FN3O4) contains a steroid core, an amide group, a hydroxyl group, an ether linkage, fluorine/chlorine substitution. It is notably hygroscopic, halogen substitution raises its density and boiling point, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), (2′R,3S,4′S,5′R)-6-Chloro-4′-(3-chloro-2-fluorophenyl)-N-[(3S)-3,4-dihydroxybutyl]-2′-(2,2-dimethylpropyl)-1,2-dihydro-2-oxospiro[3H-indole-3,3′-pyrrolidine]-5′-carboxamide meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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