1H-Pyrazolo[3,4-d]pyrimidin-3(2H)-one CAS 90993-87-6

Description

The molecular framework of 1H-Pyrazolo[3,4-d]pyrimidin-3(2H)-one (C5H4N4O) is characterized by an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

1H-Pyrazolo[3,4-d]pyrimidin-3(2H)-one (CAS 90993-87-6), molecular formula C5H4N4O and molecular weight 136.11 g/mol, is a CATO reference standard with documented purity and traceability.

Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.

As a certified reference material (CRM), 1H-Pyrazolo[3,4-d]pyrimidin-3(2H)-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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