2-Amino-4-oxo-1-azaspiro[4.5]dec-2-ene-3-carbonitrile CAS 929974-38-9

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

2-Amino-4-oxo-1-azaspiro[4.5]dec-2-ene-3-carbonitrile (CAS 929974-38-9), molecular formula C10H13N3O and molecular weight 191.23 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 2-Amino-4-oxo-1-azaspiro[4.5]dec-2-ene-3-carbonitrile (C10H13N3O) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

As a certified reference material (CRM), 2-Amino-4-oxo-1-azaspiro[4.5]dec-2-ene-3-carbonitrile meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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