| CAS No. | 93397-90-1 |
| Molecular Formula | C20H18N2O3 |
| Molecular Weight | 334.37 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Compared with other compounds in its class, the molecular weight of 334.37 g/mol gives 9-(Diethylamino)-3-hydroxy-5H-benzo[a]phenoxazin-5-one distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
9-(Diethylamino)-3-hydroxy-5H-benzo[a]phenoxazin-5-one (CAS 93397-90-1), molecular formula C20H18N2O3 and molecular weight 334.37 g/mol, is a CATO reference standard with documented purity and traceability.
Owing to a steroid core, an amide group, a hydroxyl group, an ether linkage, 9-(Diethylamino)-3-hydroxy-5H-benzo[a]phenoxazin-5-one is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 334.37 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 9-(Diethylamino)-3-hydroxy-5H-benzo[a]phenoxazin-5-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C20H18N2O3 |
| Molecular Weight | 334.37 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 13.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1983 |
| Nitrogen Atoms | 2 |