5-(4-Nitrophenyl)-4H-1,2,4-triazole-3-carboxylic acid CAS 944897-44-3

Description

Compared with other compounds in its class, the molecular weight of 234.17 g/mol gives 5-(4-Nitrophenyl)-4H-1,2,4-triazole-3-carboxylic acid distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

5-(4-Nitrophenyl)-4H-1,2,4-triazole-3-carboxylic acid (CAS 944897-44-3) is a high-purity analytical reference standard with the molecular formula C9H6N4O4 and a molecular weight of 234.17 g/mol.

The molecular framework of 5-(4-Nitrophenyl)-4H-1,2,4-triazole-3-carboxylic acid (C9H6N4O4) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 5-(4-Nitrophenyl)-4H-1,2,4-triazole-3-carboxylic acid meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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