5,7-Bis(1-methyl-1h-pyrazol-5-yl)pyrazolo[1,5-a]pyrimidine-2-carboxylic acid CAS 1004727-44-9

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 5,7-Bis(1-methyl-1h-pyrazol-5-yl)pyrazolo[1,5-a]pyrimidine-2-carboxylic acid.

CATO supplies 5,7-Bis(1-methyl-1h-pyrazol-5-yl)pyrazolo[1,5-a]pyrimidine-2-carboxylic acid (CAS 1004727-44-9), a certified reference material (molecular formula C15H13N7O2, MW 323.31 g/mol) for analytical method development and quality control.

The molecular framework of 5,7-Bis(1-methyl-1h-pyrazol-5-yl)pyrazolo[1,5-a]pyrimidine-2-carboxylic acid (C15H13N7O2) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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