5-((2-Chloro-4-nitrophenyl)amino)-1,3-dihydro-2H-benzo[d]imidazol-2-one CAS 1090790-03-6

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 5-((2-Chloro-4-nitrophenyl)amino)-1,3-dihydro-2H-benzo[d]imidazol-2-one.

5-((2-Chloro-4-nitrophenyl)amino)-1,3-dihydro-2H-benzo[d]imidazol-2-one (CAS 1090790-03-6), molecular formula C13H9ClN4O3 and molecular weight 304.69 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 5-((2-Chloro-4-nitrophenyl)amino)-1,3-dihydro-2H-benzo[d]imidazol-2-one (C13H9ClN4O3) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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