4-((9H-Fluoren-9-yl)methyl) 1-(tert-butyl) 2-hydroxypiperazine-1,4-dicarboxylate CAS 1228675-21-5

Purity 97%+In StockPharmaceutical Standards
CAS No.1228675-21-5
Molecular FormulaC24H28N2O5
Molecular Weight424.49 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

4-((9H-Fluoren-9-yl)methyl) 1-(tert-butyl) 2-hydroxypiperazine-1,4-dicarboxylate CAS 1228675-21-5 chemical structure

Description

4-((9H-Fluoren-9-yl)methyl) 1-(tert-butyl) 2-hydroxypiperazine-1,4-dicarboxylate (C24H28N2O5) contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

4-((9H-Fluoren-9-yl)methyl) 1-(tert-butyl) 2-hydroxypiperazine-1,4-dicarboxylate (CAS 1228675-21-5), molecular formula C24H28N2O5 and molecular weight 424.49 g/mol, is a CATO reference standard with documented purity and traceability.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

Molecular FormulaC24H28N2O5
Molecular Weight424.49 g/mol
AppearancePale yellow to yellow crystalline powder
Purity97%+
StorageStore in a cool place (≤25°C), protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)12.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1990
Nitrogen Atoms2

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