3β,5α,6β-Trihydroxycholestane CAS 1253-84-5

Description

The occurrence of 3β,5α,6β-Trihydroxycholestane is closely related to a steroid core, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

3β,5α,6β-Trihydroxycholestane (CAS 1253-84-5) is a high-purity analytical reference standard with the molecular formula C27H48O3 and a molecular weight of 420.67 g/mol.

Based on its molecular formula C27H48O3, 3β,5α,6β-Trihydroxycholestane contains a steroid core, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 420.67 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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