(7α)-7-tert-Butyldimethylsilyloxy-cholest-5-en-3-one CAS 14169-76-7

Description

The molecular framework of (7α)-7-tert-Butyldimethylsilyloxy-cholest-5-en-3-one (C33H58O2Si) is characterized by a carboxylic acid group, a hydroxyl group, an ether linkage, an aromatic ring system. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

(7α)-7-tert-Butyldimethylsilyloxy-cholest-5-en-3-one (CAS 14169-76-7), molecular formula C33H58O2Si and molecular weight 514.90 g/mol, is a CATO reference standard with documented purity and traceability.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), (7α)-7-tert-Butyldimethylsilyloxy-cholest-5-en-3-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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