5-Nitro-3-phenyl-1H-indole CAS 14182-35-5

Description

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

5-Nitro-3-phenyl-1H-indole (CAS 14182-35-5) is a high-purity analytical reference standard with the molecular formula C14H10N2O2 and a molecular weight of 238.24 g/mol.

The molecular framework of 5-Nitro-3-phenyl-1H-indole (C14H10N2O2) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

Physical & Chemical Properties

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