Ethyl 5-amino-1-(2-nitrophenyl)-1H-pyrazole-4-carboxylate CAS 16459-33-9

Description

The occurrence of Ethyl 5-amino-1-(2-nitrophenyl)-1H-pyrazole-4-carboxylate is closely related to an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

CATO supplies Ethyl 5-amino-1-(2-nitrophenyl)-1H-pyrazole-4-carboxylate (CAS 16459-33-9), a certified reference material (molecular formula C12H12N4O4, MW 276.25 g/mol) for analytical method development and quality control.

Ethyl 5-amino-1-(2-nitrophenyl)-1H-pyrazole-4-carboxylate (C12H12N4O4) contains an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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