| CAS No. | 18213-77-9 |
| Molecular Formula | C5H5N3O4 |
| Molecular Weight | 171.11 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Compared with other compounds in its class, the molecular weight of 171.11 g/mol gives 1-Methyl-5-nitro-1H-pyrazole-4-carboxylic acid distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
1-Methyl-5-nitro-1H-pyrazole-4-carboxylic acid (CAS 18213-77-9) is a high-purity analytical reference standard with the molecular formula C5H5N3O4 and a molecular weight of 171.11 g/mol.
Based on its molecular formula C5H5N3O4, 1-Methyl-5-nitro-1H-pyrazole-4-carboxylic acid contains a carbohydrate-derived structure, an amide group; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 171.11 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 1-Methyl-5-nitro-1H-pyrazole-4-carboxylic acid meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C5H5N3O4 |
| Molecular Weight | 171.11 g/mol |
| Appearance | Off-white to pale yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, ethanol, DMSO, water |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 5.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1962 |
| Nitrogen Atoms | 3 |