4-Hydroxy-5-methyl-2-(methylthio)pyrimidine CAS 20651-30-3

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

4-Hydroxy-5-methyl-2-(methylthio)pyrimidine (CAS 20651-30-3) is a high-purity analytical reference standard with the molecular formula C6H6N2OS and a molecular weight of 154.19 g/mol.

The molecular framework of 4-Hydroxy-5-methyl-2-(methylthio)pyrimidine (C6H6N2OS) is characterized by an amide group, sulfur-containing functionality, a hydroxyl group, an ether linkage, an aromatic ring system. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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