| CAS No. | 254-82-0 |
| Molecular Formula | C6H4N4 |
| Molecular Weight | 132.12 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Compared with other compounds in its class, the molecular weight of 132.12 g/mol gives Pyrimido[5,4-d]pyrimidine distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
Pyrimido[5,4-d]pyrimidine (CAS 254-82-0), molecular formula C6H4N4 and molecular weight 132.12 g/mol, is a CATO reference standard with documented purity and traceability.
Owing to a nitrile group, an aromatic ring system, Pyrimido[5,4-d]pyrimidine is typically off-white to pale yellow liquid. Its solid-state physicochemical attributes — including a molecular weight of 132.12 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
| Molecular Formula | C6H4N4 |
| Molecular Weight | 132.12 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 7.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |
| Nitrogen Atoms | 4 |