(2S,4R)-1-(((9H-Fluoren-9-yl)methoxy)carbonyl)-4-((tert-butoxycarbonyl)amino)pyrrolidine-2-carboxylic acid CAS 273222-06-3
| CAS No. | 273222-06-3 |
| Molecular Formula | C25H28N2O6 |
| Molecular Weight | 452.50 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.
(2S,4R)-1-(((9H-Fluoren-9-yl)methoxy)carbonyl)-4-((tert-butoxycarbonyl)amino)pyrrolidine-2-carboxylic acid (CAS 273222-06-3), molecular formula C25H28N2O6 and molecular weight 452.50 g/mol, is a CATO reference standard with documented purity and traceability.
Based on its molecular formula C25H28N2O6, (2S,4R)-1-(((9H-Fluoren-9-yl)methoxy)carbonyl)-4-((tert-butoxycarbonyl)amino)pyrrolidine-2-carboxylic acid contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 452.50 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C25H28N2O6 |
| Molecular Weight | 452.50 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 13.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 2 |
Recommended Standards
- Alloc-Lys(Fmoc)-OH (CAS 186350-56-1)
- (3R,4S)-4-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-1-(tert-butoxycarbonyl)pyrrolidine-3-carboxylic acid (CAS 267230-44-4)
- Fmoc-N-Me-Orn(Alloc)-OH (CAS 1858224-14-2)
- (3S,4R)-1-[(tert-Butoxy)carbonyl]-4-{[(9H-fluoren-9-ylmethoxy)carbonyl]amino}pyrrolidine-3-carboxylic acid (CAS 346610-79-5)
- 2-Acetamido-3-(4-nitrophenyl)acrylic acid (CAS 70973-01-2)
- 3-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)picolinonitrile (CAS 1150561-70-8)
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- 1-(5-Fluoro-2-iodophenyl)ethanol (CAS 1454848-01-1)
- (1R,2R)-6'-Chloro-2',3'-dihydrospiro[cyclopropane-1,1'-indene]-2-carboxylic acid (CAS 2839736-15-9)
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- 4-Bromo-N-(1h-imidazol-2-ylmethyl)-2,6-dimethylaniline (CAS 1247827-56-0)
- D-Lys16-Tirzepatide (CAS CATO-CPTD788918)
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