4,6-dimethyl-1,2-dihydro-3H-pyrazolo[3,4-b]pyridin-3-one CAS 28491-67-0

Purity 97%+In StockPharmaceutical Standards
CAS No.28491-67-0
Molecular FormulaC8H9N3O
Molecular Weight163.18 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

4,6-dimethyl-1,2-dihydro-3H-pyrazolo[3,4-b]pyridin-3-one CAS 28491-67-0 chemical structure

Description

The occurrence of 4,6-dimethyl-1,2-dihydro-3H-pyrazolo[3,4-b]pyridin-3-one is closely related to a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

CATO supplies 4,6-dimethyl-1,2-dihydro-3H-pyrazolo[3,4-b]pyridin-3-one (CAS 28491-67-0), a certified reference material (molecular formula C8H9N3O, MW 163.18 g/mol) for analytical method development and quality control.

The molecular framework of 4,6-dimethyl-1,2-dihydro-3H-pyrazolo[3,4-b]pyridin-3-one (C8H9N3O) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

CATO manufactures 4,6-dimethyl-1,2-dihydro-3H-pyrazolo[3,4-b]pyridin-3-one under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.

Physical & Chemical Properties

Molecular FormulaC8H9N3O
Molecular Weight163.18 g/mol
AppearanceOff-white to pale yellow crystalline powder. (faint amine-like odor)
Purity97%+
StorageStore at 2-8°C, protect from light
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityLight-sensitive; avoid direct strong light
Degree of Unsaturation (DBE)6.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1965
Nitrogen Atoms3

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