N'-[(1Z)-(4-Chlorophenyl)methylidene]-1'-(4-nitrophenyl)-3a,4,8,8a-tetrahydrospiro[[1,3]dioxepino[5,6-d][1,2]oxazole-6,4'-piperidine]-3-carbohydrazide CAS 303152-85-4
| CAS No. | 303152-85-4 |
| Molecular Formula | C24H24ClN5O6 |
| Molecular Weight | 513.93 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
N'-[(1Z)-(4-Chlorophenyl)methylidene]-1'-(4-nitrophenyl)-3a,4,8,8a-tetrahydrospiro[[1,3]dioxepino[5,6-d][1,2]oxazole-6,4'-piperidine]-3-carbohydrazide (CAS 303152-85-4) is a high-purity analytical reference standard with the molecular formula C24H24ClN5O6 and a molecular weight of 513.93 g/mol.
Structurally, N'-[(1Z)-(4-Chlorophenyl)methylidene]-1'-(4-nitrophenyl)-3a,4,8,8a-tetrahydrospiro[[1,3]dioxepino[5,6-d][1,2]oxazole-6,4'-piperidine]-3-carbohydrazide (C24H24ClN5O6) contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. The molecule exhibits a degree of unsaturation (DBE) of 15.0, corresponding to approximately 15 ring(s) or double-bond equivalent(s). With a molecular weight of 513.93 g/mol, it falls into the moderately-high-molecular-weight range. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C24H24ClN5O6 |
| Molecular Weight | 513.93 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor) |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 15.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 5 |
Recommended Standards
- Seco-Duocarmycin SA (CAS 152785-82-5)
- 4-Chlorobenzyl 4-(4-((2-oxo-2,3-dihydrobenzo[d]oxazol-6-yl)carbamoyl)thiazol-2-yl)piperazine-1-carboxylate (CAS 1141394-87-7)
- Flucloxacillin Monohydrate EP Impurity G (CAS CATO-C4X-164710)
- Ethyl 5-((4-chloro-N-(phenoxycarbonyl)phenyl)sulfonamido)-2-methylbenzofuran-3-carboxylate (CAS 397287-73-9)
- 3,4-Dihydro-1h-2-benzothiopyran-1-carboxylic acid (CAS 93689-97-5)
- Tert-butyl 2-(((tert-butyldimethylsilyl)oxy)methyl)-3-oxopiperidine-1-carboxylate (CAS 2095241-21-5)
- (1S)-1-[4-(2-methylpropoxy)phenyl]ethan-1-amine, acetic acid (CAS 1807938-90-4)
- Sucralfate (Technical Grade) (CAS 54182-58-0)
- (-)-Catechin gallate (CAS 130405-40-2)
- 2-(chloromethyl)-5-ethoxypyridine hydrochloride (CAS 133238-81-0)
- 5-Cyclopropoxy-N,N-dimethylpicolinamide (CAS 1243390-54-6)
- 6,7-Dimethoxyquinazoline-4-carboxylic Acid (CAS 122234-85-9)
- Gossypetin 3-methylether (CAS 86749-51-1)
- GnRH Associated Peptide (GAP) (1-13), human (CAS 100111-07-7)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com