5-(2-((3-Chloro-5-(trifluoromethyl)pyridin-2-yl)amino)ethyl)-2,3-diphenyltetrahydro-4H-pyrrolo[3,4-d]isoxazole-4,6(5H)-dione CAS 321391-93-9
| CAS No. | 321391-93-9 |
| Molecular Formula | C25H20ClF3N4O3 |
| Molecular Weight | 516.90 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Compared with other compounds in its class, the molecular weight of 516.90 g/mol gives 5-(2-((3-Chloro-5-(trifluoromethyl)pyridin-2-yl)amino)ethyl)-2,3-diphenyltetrahydro-4H-pyrrolo[3,4-d]isoxazole-4,6(5H)-dione distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
CATO supplies 5-(2-((3-Chloro-5-(trifluoromethyl)pyridin-2-yl)amino)ethyl)-2,3-diphenyltetrahydro-4H-pyrrolo[3,4-d]isoxazole-4,6(5H)-dione (CAS 321391-93-9), a certified reference material (molecular formula C25H20ClF3N4O3, MW 516.90 g/mol) for analytical method development and quality control.
5-(2-((3-Chloro-5-(trifluoromethyl)pyridin-2-yl)amino)ethyl)-2,3-diphenyltetrahydro-4H-pyrrolo[3,4-d]isoxazole-4,6(5H)-dione (C25H20ClF3N4O3) contains a steroid core, an amide group, a hydroxyl group, an ether linkage, fluorine/chlorine substitution. It is notably hygroscopic, halogen substitution raises its density and boiling point, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 5-(2-((3-Chloro-5-(trifluoromethyl)pyridin-2-yl)amino)ethyl)-2,3-diphenyltetrahydro-4H-pyrrolo[3,4-d]isoxazole-4,6(5H)-dione meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C25H20ClF3N4O3 |
| Molecular Weight | 516.90 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor, low volatility) |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 16.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Halogen Content | F/Cl-containing |
| Nitrogen Atoms | 4 |
Related Chloro Standards
- (2Z)-3-[4-(2-{4-[3-chloro-5-(trifluoromethyl)pyridin-2-yl]piperazin-1-yl}ethoxy)phenyl]-1-(pyridin-4-yl)prop-2-en-1-one (CAS 338406-46-5)
- Methyl 2-(2-(4-chloro-2-methylphenoxy)acetamido)-5-((4-ethoxyphenyl)carbamoyl)-4-methylthiophene-3-carboxylate (CAS 505096-47-9)
- 6-(1-(3-Chloro-5-(trifluoromethyl)pyridin-2-yl)-1H-pyrazol-5-yl)-4-methyl-2-(3-(trifluoromethyl)phenyl)-1,2,4-triazine-3,5(2H,4H)-dione (CAS 477709-55-0)
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Recommended Standards
- 3-((4-Bromobenzyl)thio)-5-(((4-chlorobenzyl)thio)methyl)-4-phenyl-4H-1,2,4-triazole (CAS 344269-41-6)
- c-Kit-IN-3 (CAS 2363169-01-9)
- 9-Bromo-5,6-dihydro-2,3-diiodo-imidazo[1,2-d][1,4]benzoxazepine (CAS 1282516-68-0)
- (3β)-3-O-tert-Butyldimethylsilyl-cholest-5-ene-3,24-diol (CAS 84529-86-2)
- 1-(1H-1,2,4-Triazol-5-yl)ethanone (CAS 153334-29-3)
- 2-Hydroxy-5-Nitro-N-(4-Phenyl-2-Thiazolyl) Benzamide (CAS 74675-84-6)
- Cis-3-phenyl-5-(4-(2-(pyrrolidin-1-yl)ethoxy)phenyl)cyclohexanone (CAS 88362-83-8)
- 2-Amino-5-hydroxybenzonitrile (CAS 116423-58-6)
- (4aR,7aS)-rel-Hexahydro-2H-[1,4]dioxino[2,3-c]pyrrole (CAS 694439-02-6)
- N-(4-Bromo-2-methylphenyl)-2-((5-((4-chlorobenzyl)thio)-1,3,4-thiadiazol-2-yl)thio)acetamide (CAS 476484-61-4)
- tert-butyl 4'-oxo-3',4'-dihydro-1H,1'H-spiro[piperidine-4,2'-quinazoline]-1-carboxylate (CAS 1000356-55-7)
- 1-Chloro-2-fluoro-4-(trifluoromethoxy)benzene (CAS 1404194-19-9)
- 5-(3,3-Difluoropyrrolidin-1-yl)picolinaldehyde (CAS 1707605-06-8)
- Semaglutide Sodium Salt (CAS CATO-C4X-222620)
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