1-(4-Chlorophenyl)-5-methyl-N-(p-tolyl)-1H-1,2,4-triazole-3-carboxamide CAS 321431-25-8

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

1-(4-Chlorophenyl)-5-methyl-N-(p-tolyl)-1H-1,2,4-triazole-3-carboxamide (CAS 321431-25-8), molecular formula C17H15ClN4O and molecular weight 326.78 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 1-(4-Chlorophenyl)-5-methyl-N-(p-tolyl)-1H-1,2,4-triazole-3-carboxamide (C17H15ClN4O) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

As a certified reference material (CRM), 1-(4-Chlorophenyl)-5-methyl-N-(p-tolyl)-1H-1,2,4-triazole-3-carboxamide meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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