Methyl 3-(2-chloro-6-fluorophenyl)-5-(3-(ethoxycarbonyl)-1-(4-fluorophenyl)-1H-pyrazol-4-yl)isoxazole-4-carboxylate CAS 321571-29-3
| CAS No. | 321571-29-3 |
| Molecular Formula | C23H16ClF2N3O5 |
| Molecular Weight | 487.84 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Methyl 3-(2-chloro-6-fluorophenyl)-5-(3-(ethoxycarbonyl)-1-(4-fluorophenyl)-1H-pyrazol-4-yl)isoxazole-4-carboxylate (CAS 321571-29-3), molecular formula C23H16ClF2N3O5 and molecular weight 487.84 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of Methyl 3-(2-chloro-6-fluorophenyl)-5-(3-(ethoxycarbonyl)-1-(4-fluorophenyl)-1H-pyrazol-4-yl)isoxazole-4-carboxylate (C23H16ClF2N3O5) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage, fluorine/chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
CATO manufactures Methyl 3-(2-chloro-6-fluorophenyl)-5-(3-(ethoxycarbonyl)-1-(4-fluorophenyl)-1H-pyrazol-4-yl)isoxazole-4-carboxylate under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C23H16ClF2N3O5 |
| Molecular Weight | 487.84 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor, low volatility) |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 16.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Halogen Content | F/Cl-containing |
| Nitrogen Atoms | 3 |
Related Methyl Standards
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Recommended Standards
- (4AR,6R,7R,7aS)-6-(6-amino-8-((4-chlorophenyl)thio)-9H-purin-9-yl)-7-hydroxy-2-mercaptotetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinine 2-oxide (CAS 129693-13-6)
- 7-(Chlorodifluoromethyl)-3-(phenylsulfonyl)-5-(4-(trifluoromethyl)phenyl)pyrazolo[1,5-a]pyrimidine (CAS 685107-54-4)
- (E)-2-((4-chlorobenzyl)thio)quinoline-3-carbaldehyde O-(3,4-dichlorobenzyl) oxime (CAS 478079-34-4)
- Cefaclor Impurity 61 (CAS CATO-C4X-127061)
- 2-Amino-1-(3,4-difluorophenyl)propan-1-ol (CAS 1038241-65-4)
- Cyhalofop DP (CAS 122008-86-0)
- 2-(4-Methoxyphenyl)-6-methyl-2,3-dihydro-1H-imidazo[1,2-b]pyrazole-7-carboxylic acid (CAS 1707378-79-7)
- N-Methyl-6-(1H-pyrazol-1-yl)nicotinamide (CAS 1001659-25-1)
- 6-((2-Hydroxyethyl)thio)-1,2,4-triazine-3,5-diol (CAS 300814-18-0)
- 3-Chloro-2-cyclobutoxyaniline (CAS 1249711-25-8)
- N-(p-Tolyl)-1H-imidazole-4-sulfonamide (CAS 58768-25-5)
- 4,7,7-Trimethyl-N-(5-methylisoxazol-3-yl)-3-oxobicyclo[2.2.1]heptane-1-carboxamide (CAS 618401-96-0)
- (S)-Ethyl 6-(bromomethyl)-4-methyl-2-oxo-1,2,3,4-tetrahydropyrimidine-5-carboxylate (CAS 105357-17-3)
- Endo-38a-Pro-Exenatide trifluoroacetate salt (CAS 1678416-95-9)
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