N-(2-(3-chloro-5-(trifluoromethyl)pyridin-2-yl)-2-methylhydrazine-1-carbonyl)-N-(4-fluorobenzyl)-5-methyl-1H-pyrazole-4-carboxamide CAS 321574-35-0
| CAS No. | 321574-35-0 |
| Molecular Formula | C20H17ClF4N6O2 |
| Molecular Weight | 484.83 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
CATO supplies N-(2-(3-chloro-5-(trifluoromethyl)pyridin-2-yl)-2-methylhydrazine-1-carbonyl)-N-(4-fluorobenzyl)-5-methyl-1H-pyrazole-4-carboxamide (CAS 321574-35-0), a certified reference material (molecular formula C20H17ClF4N6O2, MW 484.83 g/mol) for analytical method development and quality control.
The molecular framework of N-(2-(3-chloro-5-(trifluoromethyl)pyridin-2-yl)-2-methylhydrazine-1-carbonyl)-N-(4-fluorobenzyl)-5-methyl-1H-pyrazole-4-carboxamide (C20H17ClF4N6O2) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage, fluorine/chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C20H17ClF4N6O2 |
| Molecular Weight | 484.83 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor, low volatility) |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 13.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Halogen Content | F/Cl-containing |
| Nitrogen Atoms | 6 |
Related N Standards
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Recommended Standards
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- 1242932-32-6 2-Bromo-N-(4-methylbenzyl)butanamide MFCD03030366 (CAS 1242932-32-6)
- (3aR,7aR)-1,3-Dibenzyloctahydro-1H-benzo[d][1,3,2]diazaphosphole 2-oxide (CAS 162681-28-9)
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