4-((4'-Nitro-[1,1'-biphenyl]-4-yl)amino)-4-oxobut-2-enoic acid CAS 328540-83-6

Purity 97%+In StockPharmaceutical Standards
CAS No.328540-83-6
Molecular FormulaC16H12N2O5
Molecular Weight312.28 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

4-((4'-Nitro-[1,1'-biphenyl]-4-yl)amino)-4-oxobut-2-enoic acid CAS 328540-83-6 chemical structure

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

4-((4'-Nitro-[1,1'-biphenyl]-4-yl)amino)-4-oxobut-2-enoic acid (CAS 328540-83-6), molecular formula C16H12N2O5 and molecular weight 312.28 g/mol, is a CATO reference standard with documented purity and traceability.

Structurally, 4-((4'-Nitro-[1,1'-biphenyl]-4-yl)amino)-4-oxobut-2-enoic acid (C16H12N2O5) contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage. The molecule exhibits a degree of unsaturation (DBE) of 12.0, corresponding to approximately 12 ring(s) or double-bond equivalent(s). With a molecular weight of 312.28 g/mol, it falls into the moderately-high-molecular-weight range. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

CATO manufactures 4-((4'-Nitro-[1,1'-biphenyl]-4-yl)amino)-4-oxobut-2-enoic acid under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.

Physical & Chemical Properties

Molecular FormulaC16H12N2O5
Molecular Weight312.28 g/mol
AppearancePale yellow to yellow crystalline powder
Purity97%+
StorageStore at 2-8°C, protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)12.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)2025
Nitrogen Atoms2

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