3348-03-6 6-Carboxyfluorescein diacetate Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-6-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo- Spiro(phthalan-1,9'-xanthene)-6-carboxylic acid, 3',6'-dihydroxy-3-oxo-, diacetate 6-Carboxy-3',6'-diacetylfluorescein 6-Cfda CAS 3348-03-6
| CAS No. | 3348-03-6 |
| Molecular Formula | C25H34O9 |
| Molecular Weight | 478.53 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The molecular framework of 3348-03-6 6-Carboxyfluorescein diacetate Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-6-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo- Spiro(phthalan-1,9'-xanthene)-6-carboxylic acid, 3',6'-dihydroxy-3-oxo-, diacetate 6-Carboxy-3',6'-diacetylfluorescein 6-Cfda (C25H34O9) is characterized by a flavonoid core, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the polarity and ionization state of its functional groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
CATO supplies 3348-03-6 6-Carboxyfluorescein diacetate Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-6-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo- Spiro(phthalan-1,9'-xanthene)-6-carboxylic acid, 3',6'-dihydroxy-3-oxo-, diacetate 6-Carboxy-3',6'-diacetylfluorescein 6-Cfda (CAS 3348-03-6), a certified reference material (molecular formula C25H34O9, MW 478.53 g/mol) for analytical method development and quality control.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 3348-03-6 6-Carboxyfluorescein diacetate Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-6-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo- Spiro(phthalan-1,9'-xanthene)-6-carboxylic acid, 3',6'-dihydroxy-3-oxo-, diacetate 6-Carboxy-3',6'-diacetylfluorescein 6-Cfda meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C25H34O9 |
| Molecular Weight | 478.53 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at room temperature, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, acetonitrile, DMSO, water (slightly soluble) |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 9.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |
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Recommended Standards
- N-[(Phenylcarbamothioyl)amino]-3-(thiophen-2-yl)-6-(trifluoromethyl)thieno[3,2-b]pyridine-2-carboxamide (CAS 478048-37-2)
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- 2-Chloro-6-(1-methanesulfonyl-piperidin-3-ylmethyl)-pyrazine (CAS 1316217-51-2)
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- Oligomycin (CAS 1404-19-9)
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- 2,2'-((4-(Dimethylamino)-1,2-phenylene)bis(sulfanediyl))diacetonitrile (CAS 139483-31-1)
- (d(CH2)51,D-Ile2,Ile4,Arg8)-Vasopressin (CAS 88686-53-7)
- Methyl (1-((4-chlorophenyl)sulfonyl)piperidin-4-yl)carbamate (CAS 478047-66-4)
- D-[Phe6]-Semaglutide (CAS CATO-C4X-2226213)
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