Methyl 5-((4-ethoxyphenyl)carbamoyl)-4-methyl-2-(2-(o-tolyloxy)acetamido)thiophene-3-carboxylate CAS 335406-41-2
| CAS No. | 335406-41-2 |
| Molecular Formula | C25H26N2O6S |
| Molecular Weight | 482.55 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
CATO supplies Methyl 5-((4-ethoxyphenyl)carbamoyl)-4-methyl-2-(2-(o-tolyloxy)acetamido)thiophene-3-carboxylate (CAS 335406-41-2), a certified reference material (molecular formula C25H26N2O6S, MW 482.55 g/mol) for analytical method development and quality control.
The molecular framework of Methyl 5-((4-ethoxyphenyl)carbamoyl)-4-methyl-2-(2-(o-tolyloxy)acetamido)thiophene-3-carboxylate (C25H26N2O6S) is characterized by a flavonoid core, an amide group, a sulfone group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
Physical & Chemical Properties
| Molecular Formula | C25H26N2O6S |
| Molecular Weight | 482.55 g/mol |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 2 |
| Sulfur Atoms | 1 |
Related Methyl Standards
- Methyl 5-((4-ethoxyphenyl)carbamoyl)-2-(2-(4-methoxyphenyl)acetamido)-4-methylthiophene-3-carboxylate (CAS 505095-44-3)
- 1-((4-Ethoxyphenyl)amino)-4-((3,3,5-trimethylcyclohexyl)amino)anthracene-9,10-dione (CAS 29205-96-7)
- 2-((4-(4-Ethoxyphenyl)-5-(pyridin-3-yl)-4H-1,2,4-triazol-3-yl)thio)-N-(2-methoxy-5-methylphenyl)acetamide (CAS 477330-65-7)
- N-(4-Ethoxyphenyl)-2-((4-(4-ethoxyphenyl)-5-(pyridin-3-yl)-4H-1,2,4-triazol-3-yl)thio)acetamide (CAS 477329-73-0)
- 2-((5-(4-Ethoxyphenyl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio)-N-(4-methoxybenzyl)acetamide (CAS 720667-83-4)
- N-(4-Ethoxyphenyl)-2-((5-(phenoxymethyl)-4-(p-tolyl)-4H-1,2,4-triazol-3-yl)thio)acetamide (CAS 333322-66-0)
- N-(2-Ethoxyphenyl)-2-((4-(4-methoxyphenyl)-5-(phenoxymethyl)-4H-1,2,4-triazol-3-yl)thio)acetamide (CAS 539808-36-1)
- N-(4-Ethoxyphenyl)-2-((4-(4-methoxyphenyl)-5-(phenoxymethyl)-4H-1,2,4-triazol-3-yl)thio)acetamide (CAS 539808-61-2)
Recommended Standards
- TD-198946 (1-methyl-8-[4-(2-quinolinylmethoxy)phenoxy]-4,5-dihydro-1h-thieno [3,4-g]indazole-6-carboxamide) (CAS 364762-86-7)
- Ethyl 2-(2-(4-methoxyphenoxy)acetamido)-4-methyl-5-(o-tolylcarbamoyl)thiophene-3-carboxylate (CAS 518350-23-7)
- Cefixime Impurity 61 (CAS CATO-C4X-113961)
- CP-481715 (CAS 212790-31-3)
- 4-Bromo-2-(difluoromethyl)-3-methoxy-5-nitropyridine (CAS 1807028-79-0)
- 3-Methyl-2(5H)-furanone (CAS 22122-36-7)
- Methyl 2-(4-(aminomethyl)phenoxy)propanoate (CAS 1304316-41-3)
- 3-Hydroxy Anagrelide (CAS 733043-41-9)
- 4-(4-(4-Fluorophenyl)-4H-1,2,4-triazol-3-yl)piperidine (CAS 1368783-42-9)
- (R)-2-Chloro-7-methyl-7,8-dihydropteridin-6(5H)-one (CAS 1435462-66-0)
- 3-Methyl-N-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pyridazine-4-carboxamide (CAS 2246741-53-5)
- 3-(tert-Butoxy)-2-(methylthio)phenol (CAS 1243474-45-4)
- tert-Butyl N-(3-chloro-4-hydroxyphenyl)-N-ethylcarbamate (CAS 1881293-22-6)
- Semaglutide impurity 77 (CAS CATO-C4X-222677)
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