4,4-Dibromo-2,4-dihydro-5-methyl-3H-pyrazol-3-one CAS 33549-66-5
| CAS No. | 33549-66-5 |
| Molecular Formula | C4H4Br2N2O |
| Molecular Weight | 255.90 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
4,4-Dibromo-2,4-dihydro-5-methyl-3H-pyrazol-3-one (CAS 33549-66-5) is a high-purity analytical reference standard with the molecular formula C4H4Br2N2O and a molecular weight of 255.90 g/mol.
The molecular framework of 4,4-Dibromo-2,4-dihydro-5-methyl-3H-pyrazol-3-one (C4H4Br2N2O) is characterized by an amide group, a hydroxyl group, an ether linkage, bromine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
As a certified reference material (CRM), 4,4-Dibromo-2,4-dihydro-5-methyl-3H-pyrazol-3-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C4H4Br2N2O |
| Molecular Weight | 255.90 g/mol |
| Appearance | White to pale yellow crystalline powder. (slight halide-like odor, faint amine-like odor, low volatility) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep tightly closed to prevent evaporation |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 3.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1966 |
| Halogen Content | Br-containing |
| Nitrogen Atoms | 2 |
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