1H,2H,3H,4H,5H,6H,7H-pyrazolo[3,4-b]pyridin-3-one CAS 3649-44-3

Purity 97%+In StockPharmaceutical Standards
CAS No.3649-44-3
Molecular FormulaC6H9N3O
Molecular Weight139.16 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

1H,2H,3H,4H,5H,6H,7H-pyrazolo[3,4-b]pyridin-3-one CAS 3649-44-3 chemical structure

Description

1H,2H,3H,4H,5H,6H,7H-pyrazolo[3,4-b]pyridin-3-one (CAS 3649-44-3) is a high-purity analytical reference standard with the molecular formula C6H9N3O and a molecular weight of 139.16 g/mol.

Based on its molecular formula C6H9N3O, 1H,2H,3H,4H,5H,6H,7H-pyrazolo[3,4-b]pyridin-3-one contains an amide group, a hydroxyl group, an ether linkage, an aromatic ring system; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 139.16 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 1H,2H,3H,4H,5H,6H,7H-pyrazolo[3,4-b]pyridin-3-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC6H9N3O
Molecular Weight139.16 g/mol
Purity97%+
StorageStore at 2-8°C, protect from light
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityLight-sensitive; avoid direct strong light
Degree of Unsaturation (DBE)4.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1958
Nitrogen Atoms3

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