6-Hydroxy-1-methyl-1,4-dihydro-5H-pyrazolo[4,3-b]pyridin-5-one CAS 382622-66-4
| CAS No. | 382622-66-4 |
| Molecular Formula | C7H7N3O2 |
| Molecular Weight | 165.15 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
6-Hydroxy-1-methyl-1,4-dihydro-5H-pyrazolo[4,3-b]pyridin-5-one (CAS 382622-66-4) is a high-purity analytical reference standard with the molecular formula C7H7N3O2 and a molecular weight of 165.15 g/mol.
Structurally, 6-Hydroxy-1-methyl-1,4-dihydro-5H-pyrazolo[4,3-b]pyridin-5-one (C7H7N3O2) contains an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. The molecule exhibits a degree of unsaturation (DBE) of 6.0, corresponding to approximately 6 ring(s) or double-bond equivalent(s). With a molecular weight of 165.15 g/mol, it falls into the medium-molecular-weight range. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
As a certified reference material (CRM), 6-Hydroxy-1-methyl-1,4-dihydro-5H-pyrazolo[4,3-b]pyridin-5-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C7H7N3O2 |
| Molecular Weight | 165.15 g/mol |
| Appearance | Off-white to pale yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 3 |
Related Hydroxy Standards
- 4-Hydroxy-6-methyl-1,2-dihydro-pyrazolo[3,4-b]pyridin-3-one (CAS 116081-20-0)
- 6-Hydroxy-4-methylbenzo[d]oxazol-2(3H)-one (CAS 1231749-32-8)
- 4-hydroxy-1-methyl-1,2-dihydro-3H-pyrazolo[3,4-b]pyridin-3-one (CAS 1305712-64-4)
- 5-Hydroxy-2H-benzo[b][1,4]oxazin-3(4H)-one (CAS 177210-33-2)
- 8-Hydroxy-3,4-dihydro-2H-1,3-benzoxazin-2-one (CAS 1779842-32-8)
- 7-Hydroxy-3,4-dihydro-2H-1,3-benzoxazin-2-one (CAS 1783742-44-8)
- 4-Hydroxy-2-methoxybenzo[d]oxazole (CAS 1804412-50-7)
- 2-Hydroxy-3,4-dihydro-2H-1,4-benzoxazin-3-one (CAS 23520-34-5)
Recommended Standards
- 6-Amino-9-methyl-3,9-dihydro-2H-purin-2-one (CAS 54746-36-0)
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- 6-Amino-5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-ol (CAS 70384-75-7)
- 6-Hydroxy-2H-1,4-Benzoxazin-3(4H)-one (CAS 53412-38-7)
- 5-Bromo-2,3-dichlorophenol (CAS 1804909-63-4)
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- (1R,3S)-3-ethoxy-2,2-dimethylcyclobutan-1-ol (CAS 2092478-30-1)
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