Ethyl 3-(5-cyano-3-(4-methylbenzyl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(4-methylbenzyl)-1H-indole-2-carboxylate CAS 400088-14-4
| CAS No. | 400088-14-4 |
| Molecular Formula | C32H28N4O4 |
| Molecular Weight | 532.59 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Compared with other compounds in its class, the molecular weight of 532.59 g/mol gives Ethyl 3-(5-cyano-3-(4-methylbenzyl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(4-methylbenzyl)-1H-indole-2-carboxylate distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
Ethyl 3-(5-cyano-3-(4-methylbenzyl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(4-methylbenzyl)-1H-indole-2-carboxylate (CAS 400088-14-4), molecular formula C32H28N4O4 and molecular weight 532.59 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of Ethyl 3-(5-cyano-3-(4-methylbenzyl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(4-methylbenzyl)-1H-indole-2-carboxylate (C32H28N4O4) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), Ethyl 3-(5-cyano-3-(4-methylbenzyl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-1-(4-methylbenzyl)-1H-indole-2-carboxylate meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C32H28N4O4 |
| Molecular Weight | 532.59 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 21.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 4 |
Related Ethyl Standards
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Recommended Standards
- Benzyl (4-(benzyloxy)-1-methyl-7-phenoxyisoquinoline-3-carbonyl)glycinate (CAS 808118-43-6)
- Cefpirome Impurity 5 (CAS CATO-C4X-12075)
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- Relugoli Impurity 145 (CAS CATO-C4X-2429145)
- (E)-({6-phenylimidazo[2,1-b][1,3]thiazol-5-yl}methylidene)amino pentanoate (CAS 338418-13-6)
- 2-(2,6-Dimethyltetrahydro-2H-pyran-4-yl)acetic acid (CAS 1215417-58-5)
- 4-[(2E)-3-(Thiophen-2-yl)prop-2-enoyl]phenyl 4-methylbenzoate (CAS 306731-98-6)
- N-(4-Acetamidophenyl)-4-(1H-tetrazol-1-yl)benzamide (CAS 483976-57-4)
- Methyl 4-chloro-2-(trifluoromethyl)pyrimidine-5-carboxylate (CAS 1393576-33-4)
- 6-(Trifluoromethyl)-1,4,5,6-tetrahydropyrimidin-2-amine dihydrochloride (CAS 760214-90-2)
- N-(4-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)morpholine-4-carboxamide (CAS 874290-97-8)
- Z-Phe-Arg-AMC · HCl (CAS CATO-CPTD786194)
- 2-Amino-4,5,6,7-tetrachloroisoindoline-1,3-dione (CAS 6641-31-2)
- His-Cyclic-Semaglutide (CAS CATO-C4X-2226182)
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